![]() ![]() Per buffer, includes analytical column equilibration, BSA control, in addtion to per-sample fee. Per sample fee, in duplicate wells, 5-10 acquisitions/scan, 2 scans/well, in addition to plate setup fee. In addition to per-sample fee.ĭLS - sample data collection - macromolecules Instruments, service and support in Australia and New Zealand provided. Setup fee per plate, up to 48 samples, includes BSA control and 1 buffer control. Dynamic Light Scattering (DLS) is an established and precise measurement. Zeta-potential is used to characterize nanoparticles surface charge, obtaining information about their stability and surface interaction with other molecules. Required for samples with high molecular weight modifiers, such as glycosylation or detergent micelle will require additional analysisĭn/dc measurements of detergent or protein samples Dynamic light scattering is used to measure nanoparticles size, but also to evaluate their stability over time in suspension, at different pH and temperature conditions. Hydrodynamic Radius from DLS (when light scattering signal is sufficient) Includes:Ĭolumn equilibration with your buffer (pH at or below pH 7.5) or with PBSīSA Standard to test column performance and normalize detectors Prior purification by size-exclusion chromatography (SEC) is strongly recommended. Size-exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS)ĬMI SEC-MALS System: Wyatt Dawn Heleos with in-line DLS, RI UV, Agilent chromatography Standard SEC-MALS Serviceįor proteins and protein complexes between 15 KDa and 1 MDa, without high molecular weight modifiers (modifiers of mass >5% total). Dynamic Light Scattering (DLS) is a commonly used term to describe a technique which measures the particle size and estimated distribution of submicron particulate systems. ![]()
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